Bioinformatically Prediction of the miRNAs Targeting BCL9,FRMD6 Genes in Colorectal Cancer

Document Type : Original Article

Authors

1 Department of Biology , Faculty of Basic Sciences , Islamic Azad University , Qaemshahr Branch , Ma-zandaran , Iran

2 Department of Biology , Faculty of Basic Sciences , Islamic Azad University , Qaemshahr Branch , Mazandaran , Iran

3 Department of Medical laboratory sciences, faculty of medical sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran.

10.22034/mbt.2021.299246.1004

Abstract

CRC is a malignancy with no significant symptoms such as bleeding or abdominal pain, and the condition is usually diagnosed when the tumor growth makes the treatment difficult. The survival of CRC patients depends on the disease progression. Most of the CRCs begin with an unbalanced and noncancerous growth of a polyp. CRC may be prevented if the polyps are removed from the colon. Screening reduces the incidence and mortality of CRC. As the miRNAs play a significant role in the regulation of the cellular procedures, this study aims to predict the miRNAs, targeting the BCL9,FRMD6 genes , using bioinformatics tools. After getting the BCL9,FRMD6 protein chain from the NCBI database, the miRNAs targeting the genes were predicted using TargetScan, miRDB, DIANA and miRWalk databases using different algorithms. Based on the scoring system of the bioinformatics software and considering the best targeting scores, (hsa-miR-30e-5p , hsa-miR-30d-5p) and (hsa-miR-106b-5p , hsa-miR-106a-5p) are suggested as the potential miRNAs, targeting the BCL9,FRMD6 genes for future researches. Considering the significant role of the BCL9,FRMD6 genes on Colorectal cancer, the predicted miRNAs can be used as the molecular biomarkers for the early detection of Colorectal cancer patients.

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