The Effect of Diclofenac Sodium on Blood Vessel Formation

Document Type: Research Paper

Authors

Department of Pharmacy, University of Islamabad, Pakistan

Abstract

The blood vessel formation known as angiogenesis is an identifying characteristic of nearly all neoplastic and non-neoplastic degenerative diseases. The angiogenesis involves in normal physiology, in the progression of various diseases, as well as in the development of progressive arthritis and tumors. It is also deduced by inflammatory mediators that are included in cancer and are furthered by cyclo-oxygenases in order to contribution in endothelial cell spreading and cell migration. The effect of diclofenac sodium on angiogenesis has been explored using in vitro Chorioallantoic membrane assay. Structural changes in CAMs were carefully assessed by an inventive image probing system of scanning probe image processor (SPIP). About the number of fourteen parameters of 3D surface roughness was also quantified. The anti-angiogenic effect was observed at the sixth day of incubation by utilizing diclofenac sodium on Chorioallantoic membrane (0.7% of diclofenac sodium concentration). As a result, there have been changes in series of parameters containing the structure of CAMs, decrease in surface roughness, thinning of primary, secondary and tertiary blood vessels, enhancement of kurtosis of the 3D surface, and reduction of Abbott curve. The influential values for the local use of diclofenac sodium would demonstrate anti-angiogenic activity same as the in-vitro condition which illustrates its clinical efficacy.  

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