Marker-Analysis of Leaf Resistance Bacterial in Rice

Document Type: Research Paper

Authors

Department of Biology, University of Dakar, Bangladesh

Abstract

This study was carried out with the aim of cultivating different types of fine-grain and high-growing rice. Moreover, due to the transfer of bacterial leaf blight (BLB) resistant genes, such as xa13, viz., and Xa21 from B95-1, they take advantage of Broad-spectrum resistance and durable resistance. MTU1010 (Cottondora Sannalu), as a commonly used fine-grain and high-growing rice, was chosen as the parent in the crossing procedure. In addition, this particular variety is quite vulnerable to BLB and B95-1 bearing resistant genes for BLB (Xa21 & xa13 genes). Using gene-associated primers viz., xa13 promotor and pTA 248, an experiment was carried out which proved that the target genes were widely present in B95-1. Furthermore, in order to study polymorphism between rather vulnerable (MTU1010) and resistant (B95-1) parents, the aforementioned primers were utilized as well. During Rabi, 2010 and Kharif, 2011, the cross viz., MTU1010 x B95-1 was heavily influenced and F1 progenies were firmly established. While the F1 plants were considered as true hybrids for both of the genes which progressed to the F2 generation, gene-associated markers were implemented in order to carry out the foreground selection. The genes (xa13 & Xa21) that essentially control BLB resistance obeyed Mendelian inheritance, which was suggested in a Genetic analysis of F2 populations. Finally, the plants bearing a combination of two resistance gene (xa13xa13 Xa21Xa21, xa13xa13Xa21xa21) demonstrated BLB resistance (0-2 scale), while the combinations of genes including viz., Xa13Xa13Xa21Xa21, Xa13Xa13Xa21xa21, and Xa13xa13Xa21Xa21, Xa13xa13Xa21xa21 illustrated BLB resistance (0.5-3.0), which was proposed using the phenotypic data analysis.

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