The Bioproduction of Ethanol through Isolation of Some Local Bacteria

Document Type: Research Paper


Environmental science department Karachi University, Pakistan


The present study describes the ethanol fermentation from sugarcane molasses by locally isolated yeast strain. Ten yeast strains were isolated from soil and cultured in 15% molassess medium. Saccharomyces cerevisiae Bio-07 gave maximum productivity (52.0g/L). Fermentation conditions were optimized for maximum production of ethanol. Maximum yield of ethanol (76.8 g/L) was obtained with 15% molasses concentration, 3% inoculum size, pH 4.5 and temperature 30 ° C. Potassium Ferrocyanide (150ppm) was used to control the trace metals present in the molasses medium.