Production and Purification of Xylanase Enzyme through some Bacteria and Investigation of its Hydrolysis Effects

Document Type: Research Paper

Author

Department of Biotechnology, Faculty of Science, University of Dubai, UAE

Abstract

The Xylans are non-heterogeneous poly saccharides forming the major part of hemi cellulose in plant cell walls. Extracted from different species of microorganisms, xylanases (EC:3.2.1.8) are the enzymes capable of degrading the xylosidic bonds in the xylan backbone, producing xylose and other mono saccharides. In the present research, the Xylanase enzyme was first purified from basiculls thermophilus (ATCC12980) through sediment by ammonium solphate, gel filtration chromatography by sephadex G-100 and ion exchange chromatography by di ethyl amino ethyl-cellulose. Then, the enzyme effectiveness was studied in hydrolysis of two kinds of xylan (obtained from Betula and Avena sativa). Di ethyl amino ethyl-cellulose column chromatography was observed in three individual picks with only one pick showing Xylanase activity. The degree of purity obtained from the pick fractions was determined as 63.09.
The specific activity value of the purified enzyme was calculated as 87.7 I.U in/per milligram and its purification yield was determined as 17.45%. Through the enzyme, the hydrolysis levels of xylans from Betula and Avena sativa were 100 and 56.8 percent in 24 hours, respectively. Based on the results, it turned out that using the purified xylanase enzyme will be helpful for the industries exploiting the Betula woods.

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