Study of Genetic Diversity of Iranian Wheat through Microsatellite Marking

Authors

Department of Biotechnology, Faculty of Agriculture, Islamic Azad University Najaf Abad Branch, Iran

Abstract

Among the most effective measures in improving crops is to investigate their genetic structures. In the present study, 25 Einkorn wheat populations from two species of Triticum boeoticum and Triticum urartu collected from west and northwest provinces of Iran were investigated using 12 pairs of microsatellite markers. 12 pairs of primers multiplied 2-14 alleles and a total of 87 alleles among 25 genotypes with mean number of 7.25 alleles for each primer pair. Polymorphism information content was obtained 0.37 to 0.92 and the mean was calculated 0.72. Cluster analysis was carried out using UPGMA method and Dice and Jaccard similarity coefficients. Based on the plotted dendrograms, the genotypes of T.boeoticum and T.urartu species showed the highest diversities in Lorestan and Kermanshah provinces, respectively. This implies Lorestan and Kermanshah as the main origins of the two mentioned species. Totally, the studied populations of Einkorn Wheat plants exhibited higher diversities in the west provinces compared to the northwest ones.

Keywords