Document Type: Research Paper
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University Ilam Branch, Iran
Today, the use of biofuels has become a point of interest due to the limited fossil fuels resources. The objective of the present study was to investigate the production of Saccharomyces cerevisiae (D99), and ethanol and Pichiia fermentans (PTCC No:5296) at glucose inhibition concentrations. Samples were cultured in fermentation mediums containing concentrations of 100, 150, and 200 g/l glucose at 25 ° C. The amount of produced ethanol was measured by potassium dichromate method. The results showed that the ethanol production efficiency was achieved at 150 g/l concentration of glucose for both yeasts. In addition, the concentration of 200 g/l limited the production of ethanol.