Therapeutic effect of recombinant adenovirus on AGS cancer cell line

Yarkeh-Salkhori, Najmeh; Naserpour-Farivar, Taghi; Nowroozi, Jamileh; Najafipour, Reza; Pakzad, Parviz; Alizadeh, Safar-Ali

doi:10.22034/mbt.2021.319102.1007

Therapeutic effect of recombinant adenovirus on AGS cancer cell line

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University of Tehran North Branch, Tehran, IR Iran.

² Cellular and Molecular Research Center, Faculty of Basic Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran

³ Cellular and Molecular Research Center, Faculty of Basic Sciences, Qazvin University of Medical Sciences, Qazvin, IR Iran.

10.22034/mbt.2021.319102.1007

Abstract

Oncolytic viruses are tumor selective and potential anticancer agents. Adenovirus type 5 is the most commonly used oncolytic agent for treating tumors. The poor expression of the viral receptor on the surface of tumor cells is a major problem in the use of adenovirus type 5. The study aimed to improve the attachment of the modified adenovirus to tumor cells by applying an analog of somatostatin [SST] [i.e., octreotate]. In the adenovirus fiber knob's HI-loop, the octreotate sequence was introduced. By utilizing the calcium phosphate technique, modified virus was transmitted to HEK 293 packing cells, and a new virus was generated. Using the TCID50 technique, the virus titer was determined. The human adenocarcinoma stomach cell line was challenged with modified adenovirus and wild-type 5 strain [AGS]. Next, compounds were investigated for cytopathic impacts. The modified virus was validated employing PCR and sequencing. In comparison to the wild-type adenovirus, the modified adenovirus was found to be more capable of infecting AGS cells. Experiments showed that over 81% of AGS cells treated with fiber-modified Ad5 were killed after three to four days. This study suggests that fiber-modified Ad5 is an oncolytic agent against AGS cells.

Keywords